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Polynucleotide Suitable for Single Cell Based Reporter Assay for Monitoring Gene Expression Patterns with High Spatiotemporal Resolution
Polynucleotide Suitable for Single Cell Based Reporter Assay for Monitoring Gene Expression Patterns with High Spatiotemporal Resolution
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机译:多核苷酸适用于基于单细胞的报告基因检测,以高时空分辨率监测基因表达模式
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摘要
The invention relates to a double-stranded polynucleotide comprising on its positive strand considered from its 5' end to its 3' end, (i) a promoter of a gene of interest or several promoters of various genes of interest selected among genes which are endogenous to a determined cell, and, (ii) one or several barcode(s) wherein each barcode contains at least one barcode unit formed of at least one, especially of multiple, recognition binding sites each binding site being composed of a nucleotide sequence, and wherein each of said barcode(s) is under the control of at least one of said promoter(s) for transcription. It further concerns use of said polynucleotide to monitor gene expression patterns in living cells, especially in single cells, with a rapid and high spatio-temporal resolution.
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