Polynucleotide suitable for an indicator based assay in a single cell to control gene expression patterns with a high resolution spatiotemporal

摘要

Double-stranded polynucleotide suitable to carry out the expression assay gene profiling when integrated into the cell harboring naturally and possibly expresses the gene of interest to such profiling, comprising on its positive strand considered from its 5 'at its 3' end, (i) a promoter of a gene of interest or several promoters of various genes selected from various genes that are (a) endogenous to the cell and are (b) subjected to expression profiling interest gene, wherein said promoter is recognized by the mechanism of internal transcription of the cell and (ii) one or more barcodes in which each barcode contains at least one unit of barcode that is a montage DNA comprising tandem repeats of a binding site recognition mark or tandem repeats of at least two different binding sites recognition beacon being compos or each binding site by a nucleotide sequence that hybridizes specifically with the loop part of a molecular beacon in which the loop part has from 10 to 55 nucleotides and wherein each of said (s) code (s ) bar is (are) under the control of at least one of said (s) promoter (s) for transcription.