METHOD FOR SYNTHESIZING PROTEIN NANOPARTICLES USING WASTE CHICKEN FEATHERS

摘要

The embodiments herein disclose a method of synthesizing protein nanoparticles from waste chicken feathers by enzymatic hydrolysis followed by ultrasonic treatment. The steps for the synthesis of the protein nanoparticles include pretreatment of the chicken feathers. The next step is hydrolyzing the feather fibres enzymatically. Further the effect of the enzyme concentration, hydrolysis time and substrate concentration are analyzed. Also the protein nanoparticles are characterized. The effects of enzyme concentration, hydrolysis time, and substrate concentration on particle mean size are analyzed to optimize the best condition in order to attain the smallest particles by a Box-Behnken Design. It was found that minimum particle size can be obtained by using 5 g/l feather and 3.6% enzyme at hydrolysis time of 243 h. A validation assay confirmed the predictive response value under the optimal conditions.