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Method for synthesizing protein nanoparticles using waste chicken feathers
Method for synthesizing protein nanoparticles using waste chicken feathers
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机译:用废鸡毛合成蛋白质纳米颗粒的方法
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摘要
A method of producing protein nanoparticles from waste chicken feathers by enzymatic hydrolysis followed by ultrasonic treatment is disclosed. The process includes pretreatment of the chicken feathers. Then feather fibers are hydrolyzed enzymatically. The effects of enzyme concentration, hydrolysis time, and substrate concentration on particle mean size are analyzed to optimize the best condition in order to attain the smallest particles by a Box-Behnken Design. A minimum particle size is obtained by using 5 g/l feather and 3.6% enzyme with a hydrolysis time of 243 h. The hydrolyzed particles are then subjected to ultrasonic treatment to produce nanoparticles. The produced protein particles have high potential to be used in nanocomposites and adsorbents.
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机译:公开了一种通过酶水解然后超声处理从废鸡毛中生产蛋白质纳米颗粒的方法。该过程包括鸡羽毛的预处理。然后,羽毛纤维被酶水解。通过Box-Behnken设计,分析了酶浓度,水解时间和底物浓度对颗粒平均大小的影响,以优化最佳条件,从而获得最小的颗粒。通过使用5 g / l的羽毛和3.6%的酶,水解时间为243 h,可获得最小粒度。然后将水解的颗粒进行超声处理以产生纳米颗粒。产生的蛋白质颗粒具有用于纳米复合材料和吸附剂的高潜力。
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