METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO CARDIOMYOCYTES, AND CULTURE MEDIUM ADDITIVE, DIFFERENTIATION-INDUCTION REGULATOR, CULTURE MEDIUM, CULTURE MEDIUM PREPARATION KIT, AND KIT FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO CARDIOMYOCYTES SUITABLE FOR SAID METHOD

摘要

A method for inducing the differentiation of pluripotent stem cells into cardiomyocytes, said method characterized by comprising an embryoid body formation step, a mesodermal induction step, and a cardiomyocyte induction step, wherein: the cardiomyocyte induction step comprises a first cardiomyocyte induction process and a second cardiomyocyte induction process; the culture medium used in the embryoid body formation step is a first differentiation-induction culture medium to which a ROCK inhibitor has been added, or the like; the culture medium used in the mesodermal induction step is a second differentiation-induction culture medium to which a Wnt signal-activating substance has been added, or the like; the culture medium used in the first cardiomyocyte induction process is a second differentiation-induction culture medium to which a Wnt signal-inhibiting substance etc. has been added, or the like; the culture medium used in the second cardiomyocyte induction process is a second differentiation-induction culture medium to which an estrogenic agonist has been added, or the like; the first differentiation-induction culture medium is a basal medium to which insulin etc. has been added; and the second differentiation-induction culture medium is an Iscove's modified Dulbecco's medium to which albumin etc. has been added.