NUCLER MOELCULES FOR DETECTING UV INDUCED DAMAGE AND METHODS AND SYSTEMS FOR EVALUATING UV INDUCED DAMAGE AND REPAIRBY USING THEREOF

摘要

The present invention provides a nucleic acid molecule, a recombinant vector, and a transformant for sensing ultraviolet damage; a method and a device for evaluating ultraviolet damage and restoration by using the same; and a method and a device for searching an ultraviolet damage adjusting material. More specifically, the present invention relates to a nucleic acid molecule, a recombinant vector, and a transformant, in which a nucleotide sequence represented by SEQ ID NO: 1 and coding a polypeptide having a hypoxanthine phosphoribosyl transferase activity, a sensing sequence for generating a stop codon or a thymine dimer by a change of cyanine into thymine on a sequence, and a nucleotide sequence represented by SEQ ID NO: 2 and coding a polypeptide having a luciferase activity are sequentially arranged; a method and a device for evaluating ultraviolet damage and restoration by using the same; and a method and a device for searching an ultraviolet damage adjusting material. The present invention is excellent in overall aspects including basic principles, technical completion, sensitivity, and efficiency compared to found techniques of a conventional DNA damage adjusting material, supplements weaknesses of conventional alternative techniques, and maximizes advantages. Ultraviolet damage and restoration in human cells are easily and accurately evaluated, and an ultraviolet damage adjusting material is sorted.