STRAIN A N2155/Baikal/2013 OF MURRAIN VIRUS Aphtae epizooticae TYPE A FOR CONTROL OF ANTIGENIC AND IMMUNOGENIC ACTIVITY AND TO PRODUCE BIOPREPARATIONS FOR DIAGNOSTICS AND SPECIFIC PREVENTION OF MURRAIN TYPE A

摘要

FIELD: veterinary science.;SUBSTANCE: invention relates to veterinary virology and biotechnology and concerns new murrain virus strain Aphtae epizooticae type A family of Picornaviridae, genus Aphtovirus, deposited in collection of FGBU “VNIIZZH” under registration number murrain strain A No. 2155/Baikal/2013 (industrial). Presented strain is reproduced in monolayer swine kidney cell culture (SC), continuous kidney cell cultures of siberian mountain goat (PSGK-30), VNK-21 and IB-RS-2 during 18÷24 hours of incubation of virus yield in said cell cultures reaches levels of 6.33÷7.5 lg TCD₅₀/cm³. Multiplicity of infection (1÷10 TCD/cell) causes CPP after 5 hours, maintaining initial characteristics, when passaging in cell cultures throughout 5 passages.;EFFECT: presented strain may be used for control of antigenic and immunogenic activity and to produce biopreparations for diagnosis and specific prevention of murrain type A.;1 cl, 6 tbl, 7 ex