STRAIN A N2166/KRASNODARSKIY/2013 MURRAIN VIRUS Aphtae epizooticae TYPE A FOR ANTIGENIC AND IMMUNOGENIC ACTIVITY AND TO PRODUCE BIOPREPARATIONS FOR DIAGNOSIS AND SPECIFIC PREVENTION OF MURRAIN TYPE A

摘要

FIELD: veterinary science.;SUBSTANCE: invention relates to veterinary virology and biotechnology and concerns new murrain virus strain epizooticae type A fam. Picornaviridae, genus Aphtovirus, which is deposited in collection of FGBU “VNIISZH” under registration number murrain strain 2166/Krasnodarskiy/2013 (production, control of cattle). Presented strain is reproduced in primary trypsinated monolayer swine kidney cell culture (KC), passaged siberian mountain goat kidney cell cultures (PSGK-30), VHK-21 and IB-RS-2. During 18÷24 h of incubation virus yield in said cell cultures reaches 6.0÷7.33 lg TCD₅₀/cm³. Multiplicity of infection (1÷10 TCD/cell) causes CPP after 5 hours, maintaining initial characteristics, when passaging in cell cultures throughout 5 passages.;EFFECT: presented strain may be used for antigenic and immunogenic activity and to produce biopreparations for diagnostics and specific prevention of murrain type A.;1 cl, 6 tbl, 6 ex