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High throughput detection of molecular markers based on AFLP and high through-put sequencing
High throughput detection of molecular markers based on AFLP and high through-put sequencing
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机译:基于AFLP和高通量测序的分子标记物高通量检测
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摘要
The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular marker.
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