RECOMBINANT PLASMID DNA OF PQE-30_P36GP12_GP57, PROVIDING SYNTHESIS OF RECOMBINANT PROTEIN P36GP12 IN CELLS OF ESCHERICHIA COLI, A STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF RECOMBINANT PROTEIN P36GP12, RECOMBINANT PROTEIN P36GP12, CAPABLE TO BIND LIPOPOLYSACCHARIDES ESCHERICHIA COLI

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology, specifically to recombinant production of hybrid protein in Escherichia coli cells, and can be used for purification of biotechnological substances from bacterial lipopolysaccharides. A hybrid protein P36GP12 consisting of an N-terminal oligopeptide MRGSHHHHHHGSAN and full-length protein of short tail filaments of T4-like bacteriophage isolate. Plasmid pQE-30_P36GP12_GP57 is designed, which provides synthesis of recombinant protein P36GP12 in Escherichia coli DH5αF'/pQE-30_P36GP12_GP57 strain cells, obtained by transformation of cells Escherichia coli DH5αF' constructed plasmid.;EFFECT: invention enables to obtain a recombinant protein P36GP12, having the ability to bind lipopolysaccharides Escherichia coli.;3 cl, 6 dwg, 6 ex