ARTIFICIAL GENE STBL_RBD_TRM_SC2, CODING A BICISTRONIC STRUCTURE FORMED BY THE SARS-COV-2 CORONAVIRUS GLYCOPROTEIN S RECEPTOR-BINDING DOMAIN SEQUENCES, TRANSMEMBRANE REGION, P2A-PEPTIDE AND GLYCOPROTEIN G VSV, RECOMBINANT PLASMID PSTEM-RVSV-STBL_RBD_TRM_SC2, PROVIDING EXPRESSION OF ARTIFICIAL GENE, AND A RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS RVSV-STBL_RBD_TRM_SC2, USED TO CREATE A VACCINE AGAINST SARS-COV-2 CORONAVIRUS

摘要

FIELD: biotechnology.;SUBSTANCE: described is an artificial gene Stbl_RBD_TrM_SC2 used to create a vaccine against SARS-CoV-2 coronavirus, coding an artificial protein-immunogen, which is a bicistronic structure consisting of receptor-binding domain (RBD) sequences of SARS-CoV-2 coronavirus glycoprotein S, heterological signal peptide haemagglutinin (HA) of influenza A virus, transmembrane region, linker, P2A-peptide for splitting polyprotein during translation and glycoprotein G with mutation M(1)P(1), preventing alternative translation initiation, presented in SEQ ID NO:1 with length of 2463 bp. Described is a recombinant plasmid pStem-rVSV-Stbl_RBD_TrM_SC2, having molecular weight of 8.92⋅10⁶ dalton, size 14417 bp, presented in Fig. 2. Disclosed is a strain of rVSV-Stbl_RBD_TrM_SC2 of recombinant virus of vesicular stomatitis obtained using a recombinant plasmid pStem-rVSV-Stbl_RBD_TrM_SC2, which provides independent synthesis of coronavirus antigen (receptor-binding domain of glycoprotein S of SARS-CoV-2 with transmembrane region) and G protein of vesicular stomatitis virus, used to create a vaccine against SARS-CoV-2 coronavirus and deposited in the State collection of viral infection causative agents, rickettsioses of State Research Center of Virology and Biotechnology VECTOR of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, No. V-983.;EFFECT: technical result is improved folding of the transmembrane immunogen, higher stability of the target transgene and immunogenic / antigenic properties of the declared recombinant virus.;3 cl, 4 dwg, 2 tbl, 5 ex