ARTIFICIAL ECTOS_SC2 GENE ENCODING AN ECTODOMAIN OF THE SARS-COV-2 CORONAVIRUS S GLYCOPROTEIN WITH A C-TERMINAL TRIMERIZATION DOMAIN, A RECOMBINANT PLASMID PSTEM-RVSV-ECTOS_SC2, WHICH PROVIDES EXPRESSION OF THE ARTIFICIAL GENE, AND A RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS RVSV-ECTOS_SC2, USED TO CREATE A VACCINE AGAINST SARS-COV-2 CORONAVIRUS

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology, molecular biology, genetic engineering and medicine. What is described is an artificial gene encoding the ectodomain of SARS-CoV-2 coronavirus S glycoprotein with a C-terminal trimerization domain, having SEQ ID NO:1 of length 3723 bp. Invention also discloses a recombinant plasmid pStem-rVSV-EctoS_SC2, which provides expression of said artificial gene, presented on the physical and genetic map in Fig. 2, as well as a recombinant strain of vesicular stomatitis virus rVSV-Stbl_EctoS_SC2 for creating a vaccine against SARS-CoV-2 coronavirus, expressing antigens of SARS-CoV-2 coronavirus, inducing a specific immune response to SARS-CoV-2, deposited in the State collection of viral infections causative agents, rickettsioses of State Research Center of Virology and Biotechnology VECTOR of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, No. V-982.;EFFECT: high expression level of the transgene, improved folding and ensuring the synthesis of the full-length ectodomain of SARS-CoV-2 S glycoprotein.;3 cl, 4 dwg, 4 ex