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ITS序列作为DNA条形码在鉴定嘉兴槜李中的应用及鉴定方法

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摘要

本发明公开了ITS序列作为DNA条形码在鉴定嘉兴槜李中的应用及鉴定方法，属于分子鉴定技术领域。所述鉴定方法包括：利用PCR扩增待测样品的ITS序列并进行测序，如果ITS序列的165bp碱基为“T”、195bp碱基为“T”，则判定待测样品为嘉兴槜李。本发明还公开了一种鉴别嘉兴槜李早熟品种和晚熟品种的方法，如果ITS序列的碱基序列如SEQ ID NO.1所示，则判定待测样品为嘉兴槜李早熟品种；如果ITS序列的碱基序列如SEQ ID NO.2所示，则判定待测样品为嘉兴槜李晚熟品种。本发明利用ITS序列作为DNA条形码鉴定嘉兴槜李，检测准确性高、可重复性高，鉴定时间短。

著录项

公开/公告号CN105671185B

专利类型发明专利
公开/公告日2019-02-05

原文格式PDF
申请/专利权人浙江省嘉兴市农业科学研究院(所);
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申请/专利号CN201610186877.3
发明设计人李军;李白;李斌;
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申请日2016-03-29
分类号C12Q1/6895(20180101);C12N15/11(20060101);
代理机构33224 杭州天勤知识产权代理有限公司;
代理人沈自军
地址 314016 浙江省嘉兴市秀洲区王江泾镇双桥
入库时间 2022-08-23 10:25:12

法律信息

法律状态公告日

法律状态信息

法律状态
2019-02-05

授权

授权
2016-07-13

实质审查的生效 IPC(主分类):C12Q1/68 申请日:20160329

实质审查的生效
2016-07-13

实质审查的生效 IPC(主分类):C12Q 1/68 申请日:20160329

实质审查的生效
2016-06-15

公开

公开
2016-06-15

公开

公开

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