Analytical Variation in the Determination of the Fatty Acid Composition of Standard Preparations of Brine Shrimp 'Artemia': An Interlaboratory Exercise

摘要

An international interlaboratory exercise was conducted to investigate the variability associated with the preparation and analysis of samples and the reporting of fatty acid composition data for two samples of Artemia supplied to the laboratories by the Artemia Reference Center (ARC). Results reported to the ARC indicated that intralaboratory variability in determinations of total lipid content and fatty acid composition by the area-percent method was generally low, with a coefficient of variation (CV) around 5 to 7% on average. Intralaboratory variability in determination of fatty acid composition by the mg/g method was higher, with CVs greater than 10% in several cases. In this case, data from each of the laboratories would support the decision to use these particular Artemia only for freshwater organisms.