A guideline for the detection of selected plant viruses in forest tree seeds

摘要

We report about the transmission of plant viruses in forest tree seeds and give a guideline for the detection of viruses in deciduous forest tree seeds in general and then focuse on the detection of widespread infections caused by seedtransmissible pathogen cherry leaf roll virus (CLRV). Serological methods, dot blot hybridization and as well as a modified immunocapture RT-PCR (IC-RT-PCR) are demonstrated to detect the most described and observed seedtransmissible viruses as there are nepoviruses, ilar- cucumo- and tobamoviruses. Applied methods are valued and discussed. The application of a conserved primer pair is shown to be suitable for the detection of e.g. CLRV which was easily detectable all over the year in collected seeds collected from aCLRV-infected birch-tree.