Cytochrome c_3-Langmuir-Blodgett film for hydrogen evolving device

摘要

Cytochrome c_3 (cyt c_3) is a mediator protein for hydrogenase (H_2ase) in bacteria (Desulfovibrio sp.). Cyt c_3 should play an important role in the enzymatic electrochemical energy conversion of electricity/hydrogen using hydrogenase. In this study, a monolayer film of cyt c_3 was prepared to realize an electron mediating pathway by using the Langmuir-Blodgett (LB) technique with an amphiphilic peptide forming a complex with the protein molecule. The redox activity of the cyt c_3 LB film was confirmed by spectroscopic analysis and cyclic voltammetry. Subsequently, the cyt c_3 LB film deposited on a carbon graphite electrode was applied for the hydrogen evolution. At the applying potential, -350 mV (versus SHE), the cyt C_3 LB film on the carbon graphite electrode mediated the electron transfer from electrode to hydrogenase in solution.