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High Pressure Inactivation of Enterococcus faecium -modelling and verification

机译:粪肠球菌的高压灭活-建模与验证

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High-pressure inactivation data were obtained for model working suspensions of Enterococcus faecium in saline solutions in pH range from 5.5 to 6.8, at water activity 0.99 (corresponding to the saline solution). The data were predicted for the pressure range of 450 MPa to 550 MPa, at the initial model suspension temperature 6-7°C prior to pressurising. The results indicate that E. faecium is a highly resistant organism under physiological pH values. With decreasing the substrate pH, the tolerance tothe inactivation effects of high pressure decreased. The high-pressure inactivation proved ineffective for the above organism in the range of physiological pH values. The values for high-pressure inactivation model were specified and the calculated parameters of high-pressure inactivation were compared to the experimental data for the working suspensions of E, faecium in cow and human milks. As to cow milk, the predicted data showed some deviation from the model experimental results, while with human milk the model failed completely. High-pressure inactivation of E. faecium in human milk proved to be significantly more effective than that predicted by a model based on the saline solution.
机译:获得粪便肠球菌在pH范围为5.5至6.8,水活度为0.99(对应于盐溶液)的盐溶液中的模型工作悬浮液的高压灭活数据。在加压之前的初始模型悬浮温度为6-7°C的情况下,预测压力范围为450 MPa至550 MPa的数据。结果表明,粪肠球菌在生理pH值下是高度抗性的生物。随着底物pH值的降低,对高压灭活作用的耐受性降低。在生理pH值范围内,高压灭活被证明对上述生物无效。指定高压灭活模型的值,并将高压灭活的计算参数与牛乳和人乳中E,粪便的有效悬浮液的实验数据进行比较。对于牛奶,预测数据表明与模型实验结果有些偏差,而对于母乳,模型则完全失效。事实证明,人乳中粪肠球菌的高压灭活比基于盐溶液的模型所预测的灭活显着更有效。

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