首页> 外文期刊>Russian journal of bioorganic chemistry >Molecular environment of the subdomain IIIe loop of the RNA IRES element of hepatitis C virus on the human 40S ribosomal subunit
【24h】

Molecular environment of the subdomain IIIe loop of the RNA IRES element of hepatitis C virus on the human 40S ribosomal subunit

机译:丙型肝炎病毒RNA IRES元件在人40S核糖体亚基上IIIe环亚域的分子环境

获取原文
获取原文并翻译 | 示例
获取外文期刊封面目录资料

摘要

The molecular environment of the internal ribosome entry site (IRES element) of hepatitis C viral (HCV) RNA in the binary complex with the human 40S ribosomal subunit was studied. To this end, RNA derivatives bearing mild UV-reactive perfluorophenylazide groups at nucleotide G87 in IRES domain II and at nucleotide A296 in the subdomain IIIe loop were used, which were prepared by the RNA complementarily-addressed modification with alkylating oligonucleotide derivatives. None of the RNA derivatives were shown to be crosslinked to the 18S rRNA of the 40S subunit. It was found that the photoreactive group of IRES nucleotide A296 crosslinked to the 40S subunit S2/S3a, S5, and p40 (SOA) proteins. No protein crosslinking was observed for the RNA derivative containing the same photoreactive group at nucleotide G87. It was concluded that the subdomain IIIe loop of the HCV RNA IRES element in the complex with the 40S subunit is located on the subunit between the head and the body aside the “beak” near the exit from the mRNA-binding channel.
机译:研究了与人40S核糖体亚基二元复合体中丙型肝炎病毒(HCV)RNA的内部核糖体进入位点(IRES元件)的分子环境。为此,使用了在IRES结构域II的核苷酸G87处和在子结构域IIIe环的核苷酸A296处带有轻度UV反应性全氟苯基叠氮基的RNA衍生物,其通过用烷基化寡核苷酸衍生物对RNA进行互补寻址修饰而制备。没有显示出RNA衍生物与40S亚基的18S rRNA交联。发现IRES核苷酸A296的光反应性基团与40S亚基S2 / S3a,S5和p40(SOA)蛋白交联。对于在核苷酸G87处含有相同光反应性基团的RNA衍生物,未观察到蛋白质交联。结论是,具有40S亚基的复合物中HCV RNA IRES元件的亚域IIIe环位于mRNA与结合通道出口附近的“喙”旁边的头部和身体之间的亚基上。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号