Effect of the modification of the processing site of Bacillus intermedius glutamyl endopeptidase on the production of active enzyme by Bacillus subtilis cells

摘要

A site-directed modification at position P1 of the processing site of Bacillus intermedius glutamyl endopeptidase was carried out. Variants of the protease gene were obtained that correspond to the protein with an Ala, Asn, Ser, or Glu residue at this position substituted for the Lys residue. The residue in the P1 position of the processing site was shown to affect substantially the production of the active enzyme; however, none of the mutations leads to the complete termination of active protein production by cells.