首页> 外文期刊>Biological & pharmaceutical bulletin >Gene expression in primary cultured mouse hepatocytes with a cationic liposomal vector, TFL-3: comparison with rat hepatocytes.
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Gene expression in primary cultured mouse hepatocytes with a cationic liposomal vector, TFL-3: comparison with rat hepatocytes.

机译:带有阳离子脂质体载体TFL-3的原代培养小鼠肝细胞中的基因表达:与大鼠肝细胞的比较。

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摘要

We recently reported that a cationic liposomal vector, TFL-3, could be used to achieve significant gene expression in primary cultured rat hepatocytes (Nguyen et al., Biol. Pharm. Bull., 26, 880-885 (2003)). A combination of hepatocyte transplantation and hepatocyte-targeted gene transfer represents a potentially important strategy for expanding treatment options for liver disease. A widely applied approach to support cross-species is necessary before human applications can be realized. Therefore, in this study, we examined the utility of TFL-3 in another species of rodent hepatocytes, namely mouse hepatocytes. Gene expression in mouse hepatocytes by TFL-3 was successful and the level was higher than those in rat hepatocytes that we recently reported on. Interestingly, it appears that both the degree and rate of gene expression were dependent on the incubation time prior to lipofection as well as on the density of cells per dish, but these parameters were independent of the amount of pDNA associated with the cells. These significantly suggest that the culture time prior to and following lipofection, which are related to the biological condition of the cells, may be one of major factors that affect gene expression in hepatocytes and non- or less dividing cells.
机译:我们最近报道了阳离子脂质体载体TFL-3可用于在原代培养的大鼠肝细胞中实现显着的基因表达(Nguyen等人,Biol.Pharm.Bull。,26,880-885(2003))。肝细胞移植和肝细胞靶向基因转移的结合代表了扩大肝病治疗选择的潜在重要策略。在实现人类应用之前,必须采用广泛应用的方法来支持跨物种。因此,在这项研究中,我们检查了TFL-3在另一种啮齿动物肝细胞,即小鼠肝细胞中的效用。 TFL-3在小鼠肝细胞中成功表达了基因,并且该水平高于我们最近报道的大鼠肝细胞中的水平。有趣的是,似乎基因表达的程度和速率都取决于脂质转染前的孵育时间以及每个培养皿的细胞密度,但是这些参数与与细胞相关的pDNA的数量无关。这些明显表明,脂质转染前后的培养时间与细胞的生物学状况有关,可能是影响肝细胞和非分裂细胞或少分裂细胞中基因表达的主要因素之一。

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