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首页> 外文期刊>Radiotherapy and oncology: Journal of the European Society for Therapeutic Radiology and Oncology >Response of the plasma hypoxia marker osteopontin to in vitro hypoxia in human tumor cells.
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Response of the plasma hypoxia marker osteopontin to in vitro hypoxia in human tumor cells.

机译:血浆缺氧标志物骨桥蛋白对人肿瘤细胞体外缺氧的反应。

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BACKGROUND: Osteopontin (OPN) is recognized as a tumor-associated protein and has recently been shown to be a potential plasma marker of tumor hypoxia in head and neck cancer patients. We sought to detect patterns of OPN accumulation and secretion in human tumor cells during in vitro hypoxia. MATERIALS AND METHODS: The human tumor cell lines A 549 lung carcinoma, U 87 malignant glioma, HT 1080 fibrosarcoma, FaDu pharyngeal carcinoma and HT 29 and HCT 116 colorectal carcinoma were treated with 1, 6 or 24 h of hypoxia (0.1% O2) or 24 h followed by 4 h or 20 of reoxygenation. OPN concentration in the supernatant was measured by ELISA, OPN protein and mRNA levels by Western and Northern blotting. RESULTS: In FaDu, HT 29 and HCT 116, OPN levels in the supernatant remained below 10 ng/ml under all conditions. In A 549, HT 1080 and U 87, mean aerobic OPN concentrations were 2296, 164 and 115 ng/ml, respectively. No increase of OPN in the medium during 24h of hypoxia, but moderate increases during subsequent 24-hour-reoxygenation were observed in these three cell lines. Intracellular OPN protein was present to a similar extent in all six-cell lines under aerobic conditions and also did not accumulate during hypoxia treatment. OPN mRNA response to hypoxia and reoxygenation was very heterogeneous between cell lines. CONCLUSION: Reoxygenation rather than hypoxia appears to induce OPN secretion from human tumor cells in a cell-type specific manner.
机译:背景:骨桥蛋白(OPN)被认为是一种肿瘤相关蛋白,最近被证明是头颈癌患者肿瘤缺氧的潜在血浆标志物。我们试图检测体外缺氧期间人肿瘤细胞中OPN积累和分泌的模式。材料与方法:将人肿瘤细胞系A 549肺癌,U 87恶性神经胶质瘤,HT 1080纤维肉瘤,FaDu咽癌以及HT 29和HCT 116结直肠癌用缺氧1、6或24 h(0.1%O2)处理或24小时,然后再进行4小时或20次复氧。通过ELISA测量上清液中的OPN浓度,通过Western和Northern印迹法测量OPN蛋白和mRNA水平。结果:在FaDu,HT 29和HCT 116中,在所有条件下上清液中OPN的水平均保持低于10 ng / ml。在A 549,HT 1080和U 87中,平均有氧OPN浓度分别为2296、164和115 ng / ml。在缺氧的24小时内,培养基中的OPN没有增加,但是在这三个细胞系中,在随后的24小时的复氧过程中没有发现中等程度的增加。在有氧条件下,所有六细胞系中的细胞内OPN蛋白含量均相似,并且在缺氧治疗期间也不积累。 OPN mRNA对缺氧和复氧反应在细胞系之间非常不同。结论:复氧而非缺氧似乎以细胞类型特异性方式诱导人肿瘤细胞分泌OPN。

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