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Uncommon occurrence ratios of aflatoxin B_(l9) B_2, G_(l9) and G_2 in maize and groundnuts from Malawi

机译:马拉维玉米和花生中黄曲霉毒素B_(l9)B_2,G_(l9)和G_2的罕见发生率

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摘要

We report an unusual anatoxin profile in maize and groundnuts from Malawi, with aflatoxin G_1 found routinely at equal or even higher levels than aflatoxin B_1. Aflatoxin B_1 (AFB_1) ratio in a contaminated sample is generally greater than 50 % of total aflatoxin (sum of aflatoxin B_1, B_2, G_1, and G2). In Malawi, the aflatoxin occurrence ratios were determined by examining LC-MS/MS and HPLC fluorescence detection (FLD) data of 156 naturally contaminated raw maize and 80 groundnut samples collectedin 2011 and 2012. Results showed that natural aflatoxin occurrence ratio differed. In 47 % of the samples, the concentration of AFG[ was higher than that of AFB,. The mean concentration percentages of AFB_1/AFB_2/AFG_1/AFG_2 in reference to total aflatoxins were found to be 47:5:43:5 %, respectively. The AFG, and AFBt 50/50 trend was observed in maize and groundnuts and was consistent for samples collected in both years. If the AFB[ measurement was used to check compliance of total aflatoxin regulatorylimit set at 10, 20, 100, and 200 Hg/kg with an assumption that AFB_1>50 % of the total aflatoxin content, 8, 13, 24, and 26 % false negative rates would have occurred respectively. It is therefore important for legislation to consider total aflatoxins rather than AFB1 alone.
机译:我们报告了马拉维玉米和花生中不寻常的抗毒素特性,黄曲霉毒素G_1的水平通常等于或高于黄曲霉毒素B_1的水平。受污染样品中的黄曲霉毒素B_1(AFB_1)比例通常大于总黄曲霉毒素(黄曲霉毒素B_1,B_2,G_1和G2的总和)的50%。在马拉维,通过检查2011和2012年收集的156个自然污染的生玉米和80个花生样品的LC-MS / MS和HPLC荧光检测(FLD)数据,确定了黄曲霉毒素的发生率。结果表明,天然黄曲霉毒素的发生率存在差异。在47%的样品中,AFG [的浓度高于AFB的浓度。相对于总黄曲霉毒素,AFB_1 / AFB_2 / AFG_1 / AFG_2的平均浓度百分比分别为47:5:43:5%。在玉米和花生中观察到AFG和AFBt 50/50趋势,这与这两年收集的样品一致。如果使用AFB [测量值来检查总黄曲霉毒素法规限值是否设置为10、20、100和200 Hg / kg,则假定AFB_1>总黄曲霉毒素含量的50%,8、13、24和26%假阴性率将分别发生。因此,对于立法而言,重要的是考虑总黄曲霉毒素而不是单独使用AFB1。

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