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Temperature-dependent phase transitions in zeptoliter volumes of a complex biological membrane

机译:复杂生物膜的Zeptoliter体积中的温度依赖性相变

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摘要

Phase transitions in purple membrane have been a topic of debate for the past two decades. In this work we present studies of a reversible transition of purple membrane in the 50-60 °C range in zeptoliter volumes under different heating regimes (global heating and local heating). The temperature of the reversible phase transition is 52 ± 5 °C for both local and global heating, supporting the hypothesis that this transition is mainly due to a structural rearrangement of bR molecules and trimers. To achieve high resolution measurements of temperature-dependent phase transitions, a new scanning probe microscopy-based method was developed. We believe that our new technique can be extended to other biological systems and can contribute to the understanding of inhomogeneous phase transitions in complex systems.
机译:在过去的二十年中,紫色膜的相变一直是争论的话题。在这项工作中,我们研究了在不同的加热方式(整体加热和局部加热)下,以升升为单位的紫色膜在50-60°C范围内可逆转变的研究。对于局部和整体加热,可逆相变的温度均为52±5°C,支持以下假设:该相变主要是由于bR分子和三聚体的结构重排所致。为了实现与温度有关的相变的高分辨率测量,开发了一种新的基于扫描探针显微镜的方法。我们相信,我们的新技术可以扩展到其他生物系统,并且有助于理解复杂系统中不均匀的相变。

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