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The nucleolar protein SURF-6 is essential for viability in mouse NIH/3T3 cells

机译:核仁蛋白SURF-6对于小鼠NIH / 3T3细胞的生存力至关重要

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摘要

SURF-6 is a bona fide nucleolar protein comprising an evolutionary conserved family that extends from human to yeast. The expression of the mammalian SURF-6 has been recently found to be regulated during the cell cycle. In order to determine the importance of SURF-6 in mammalian cells, we applied the Tet-On system to regulate conditionally, in response to tetracycline, the expression of an antisense RNA (asRNA) that targets Surf-6 mRNA in mouse NIH/3T3 cells. Induced Surf-6 asRNA caused an effective depletion of SURF-6 protein resulted in cell death and in an apparent arrest in the G1 phase of the cell cycle. These results provide for the first time evidence that expression of SURF-6 is essential for mammalian cell viability, and suggest that SURF-6 might participate in the progression of cell cycle.
机译:SURF-6是真正的核仁蛋白,包含从人到酵母的进化保守家族。最近发现哺乳动物SURF-6的表达在细胞周期中受到调节。为了确定SURF-6在哺乳动物细胞中的重要性,我们应用了Tet-On系统来有条件地调节四环素,以靶向小鼠NIH / 3T3中Surf-6 mRNA的反义RNA(asRNA)的表达。细胞。诱导的Surf-6 asRNA导致SURF-6蛋白的有效耗竭,导致细胞死亡,并在细胞周期的G1期明显停滞。这些结果首次提供了SURF-6的表达对于哺乳动物细胞生存力至关重要的证据,并表明SURF-6可能参与了细胞周期的进程。

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