MOUSE HYBRIDONE S148-PRODUCER OF MONOCLONAL ANTIBODY TO NUCLEOLAR PROTEIN SURF-6 OF MAMMALS

摘要

FIELD: chemistry.;SUBSTANCE: clone of mouse hybridome cells called "S148" is obtained by merging splenic lymphocytes of a mouse which is immunised by human recombinant protein SURF-6, which is merged with glutathione S-transferase which is expressed and extracted from E. coli cells, with cells of mouse myeloma of the line P3X63-Ag8.653 in ratio of 3:1 using a 50% solution of polyethylene glycol with molecular weight 1450. The obtained hybridome secretes the monoclonal antibody S148, which is specific towards human, mouse or rat nucleolar protein SURF-6, having an established amino acid sequence.;EFFECT: disclosed monoclonal antibody S148 enables to detect nucleolar protein SURF-6 in mammal cells using immunocytochemistry, immunoblotting and immunoprecipitation techniques.;8 dwg, 7 ex