Effect of Gamma Irradiation on Fertility Restorer Sunflower Lines (Helianthus annuus L.) and Their Regeneration through Direct Organogenesis and Somatic Embryogenesis

摘要

The aim of this investigation was to follow the genotype response of five fertility restorer lines to in vitro cultivation of immature zygotic embryos using the method of direct organogen-esis and s6matic embryogenesis, as well as to study the effectof gamma- radiation at doses 2.5 Gy, 5 Gy, 7.5 Gy, lOGy, 12.5 Gy, 15Gy, 17.5 Gy, 20 Gy, 22.5 Gy and 25.0 Gy on the increasing of regeneration response. A difference was observed in the genotype response both of the check variant and the variants with gamma irradiation with respect to in vitro regeneration. The highest regeneration potential under controlled conditions was demonstrated by line K-821, and lowest - by 2128 R. Line 147 R was most sensitive to the effect of gamma-radiation. Doses 5 Gy and 10Gy proved most efficient for regeneration stimulation in genotypes 147 R and 1028 R. Lack of regeneration was registered in all investigated genotypes at dose 25 Gy, which was therefore considered terminal.