Shoot Bud and Young Leaf Induction of Jasminumspp. in in vitro Culture

摘要

In vitro culture of Jasminum sambac was cultured by shoot tip and young leaf on modification MS medium. Shoot tips were sterilized with Clorox 10, 15, 20 and 25% (v/v) for 10, 15, 20 and 25 min (minutes), respectively. It was found that Clorox 10% for10 min would be the best for surface sterilized (60%) and survived (66.66%). For young leave were surface sterile with Clorox 10% for 10 min and followed with Clorox 10% for 5 min (minutes) , which was the best surface sterilized (86.79%). After that both kind of explants were cultured on modified MS medium. Shoot tips medium was supplemented with BA 1, 2 and 4 mg/1; kinetin 1, 2 and 4 mg/1 and/or combination with NAA 0.1 mg/1 for 6 months. The results showed that shoot tips were formed shoot buds on modified MS medium supplemented with BA 4 mg/1 (50%); kinetin 1 mg/1 ofjasminum sambac strains 1 (60%), the results showed that 75% shoot budswere induced from shoot tip explants when cultured on modified MS medium supplemented with BA 4 mg/1, whereas 54.54% shoot buds were induced when cultured on modified MS medium supplemented with kinetin 1 mg/1 jasminum sambac strains 2. And modified MS medium supplemented with BA 4 mg/1 combination with NAA 0.1 mg/1 induced 2.8 shoots/explant in average and could formed callus in 18 days, while supplemented with kinetin 1 mg/1 combination with NAA 0.1 mg/1 induced 3.2 shoots/explant in average and couldformed callus in 26 days of jasminum sambac strains 1, BA 4 mg/1 combination with NAA 0.1 mg/1 could induce 2.8 shoots of jasminum sambac strains 2. For young leave were formed callus on modified MS medium supplemented with kinetin 1 mg/1 combination with 2,4-D 0.1 mg/1 and supplemented with BA 1 mg/1 in 12 days of jasminum sambac strains 1, the young leave explants were formed callus 100% within 12 days of jasminumsambac strains 2.