Production and Partial Purification of a-Amylase fromBacterial Strains

摘要

The Aim of Study is to produce alpha-Amylase by using bacterial strains namely Streptomyces cyaneus and Streptomyces diastaticus. These Enzymes have many Industrial applications and have a very wide scope. Both the bacterial species are well known forthe production of amylases. Crude enzyme was obtained from these strains. Various enzyme purification techniques used were ammonium sulfate precipitation and dialysis. Pure enzyme was obtained after dialysis and filtration. Enzyme activity and Specificactivity of enzyme was observed to be more in pure enzyme i.e. after dialysis.