首页> 外文期刊>Asian Journal of Plant Pathology >TLC Bioautography Guided Detection and Biological Activity of Antifungal Compounds from Medicinal Plant Acorus calamus Linn
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TLC Bioautography Guided Detection and Biological Activity of Antifungal Compounds from Medicinal Plant Acorus calamus Linn

机译:薄层色谱生物自传技术指导药用植物A蒲(Acorus calamus Linn)抗真菌化合物的检测和生物活性

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摘要

Plant based botanical fungicides are one of the most important alternative for hazardous fungicides. Antifungal activity (AFA), identification and understanding the mechanism of action of predominant antifungal compounds present in medicinal plant (Acorus calamus L) were carried out in this study. The antifungal activity and both n-butanol extract and purified antifungal compound of A. calamus (L) was evaluated against five fungal strains (Fusarium oxysporum, Fusarium solani, Colletotrichum gloesporioides, Botrytis cinerea and Altemaria solani) by using agar overlay techniques and bioautography. Isolation and screen of antifungal compounds (AFC) was carried out by Thin Layer Chromatography (TLC) and bioautography. The AFC were elucidated and identified by using gas chromatography-mass spectrometry. The mode of action of purified AFC from A. calamus was studied by Evans blue staining and generation of Reactive Oxygen Species (ROS). Both n-butanol extracts and purified AFC exhibited different degreesof antifungal activity against all fungal pathogens tested. Both n-butanol extract from leaf and rhizome of A calamus and purified AFC showed prominent one major spot on TLC after detection with p-anisaldehyde. In bioautographic technique, clear inhibition zones at same distance of TLC spot was observed against all fungal strains examined. The major purified antifungal compounds were identified as a p-asarone (94.3%) and a-asarone (3.4%). Fungal cells treated with different concentration of purified AFC showed a rapid production of ROS. The extent of dead cells stained with Evans blue dye seemed to correlate with the level of ROS production. These results suggested both n-butanol extracts and purified compounds from A. calamus show antifungal activityagainst plant pathogenic fungi and main compound attributed for antifungal activity is p-asarone. Furthermore, AFC caused damages to the cell membrane, leading to altering cell morphology and eventually cell death. Thus A. calamus extract has potentialapplication as an alternative or integrated method to chemical control on plant diseases caused by five different plant pathogens examined.
机译:植物性植物杀菌剂是危险杀菌剂的最重要替代品之一。在这项研究中进行了抗真菌活性(AFA),鉴定和了解药用植物(Acorus calamus L)中存在的主要抗真菌化合物的作用机理。通过使用琼脂覆盖技术和生物自显影技术,评估了cal蒲曲霉(L)的抗真菌活性以及正丁醇提取物和纯化的抗真菌化合物对五种真菌菌株(尖孢镰刀菌,枯萎镰刀菌,球形炭疽菌,灰葡萄孢和褐藻)的抗真菌活性。抗真菌化合物(AFC)的分离和筛选通过薄层色谱法(TLC)和生物自显影法进行。使用气相色谱-质谱法对AFC进行了阐明和鉴定。通过埃文斯蓝染色和活性氧(ROS)的生成研究了从cal蒲中纯化的AFC的作用方式。正丁醇提取物和纯化的AFC均对所有测试的真菌病原体表现出不同程度的抗真菌活性。用对茴香醛检测后,cal蒲的叶和根茎的正丁醇提取物以及纯化的AFC均在TLC上显示了一个重要的主要斑点。在生物自传技术中,针对所有检查的真菌菌株,在TLC点的相同距离处观察到清晰的抑制区。鉴定出主要的纯化抗真菌化合物为对伞花酮(94.3%)和对伞花酮(3.4%)。用不同浓度的纯化AFC处理的真菌细胞显示出ROS的快速产生。用伊文思蓝染料染色的死细胞的程度似乎与ROS产生的水平有关。这些结果表明正丁醇提取物和cal蒲的纯化化合物均显示出对植物病原真菌的抗真菌活性,而归因于抗真菌活性的主要化合物是对伞花龙。此外,AFC导致细胞膜受损,导致细胞形态改变,最终导致细胞死亡。因此,cal蒲提取物作为化学控制由五种不同植物病原体引起的植物病害的替代方法或综合方法具有潜在的应用前景。

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