TIME-RESOLVED FLUORESCENCE-DECAY MEASUREMENT AND ANALYSIS ON SINGLE CELLS BY FLOW CYTOMETRY

摘要

A novel method is described for the measurement and analysis of fluorescence decays of individual cells and particles in flow. It combines the rapid measurement capabilities of a flow cytometer and the robust measurement and analysis procedures of time-domain fluorescence-lifetime spectroscopy. For excitation we use a cw laser that is pulse modulated by an electro-optic modulator. The characteristics and the repetition rate of the excitation pulses can be easily adjusted to accommodate fluorescence decays with a wide range of lifetimes. (C) 1996 Optical Society of America [References: 26]