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Image formation in phase-shifting digital holography and applications to microscopy

机译:相移数字全息术中的图像形成及其在显微镜中的应用

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摘要

We discuss image formation in phase-shifting digital holography by developing an analytical formulation based on the Fresnel-Kirchhoff diffraction theory. Image-plane position and imaging magnification are derived for general configurations in which a spherical reference is employed. The influences of discrete sampling of the resulting interference patterns by a CCD and numerical reconstruction on qualities of point images are investigated. Dependence of the point images on the ratio of the minimum fringe spacing to pixel pitch of the CCD is numerically analyzed. Two-point resolution and magnification are also investigated as a function of pixel numbers by a simulation using a one-dimensional model. In experiments magnified images of biological objects and a resolution target were reconstructed with the same quality as by conventional microscopy.
机译:我们通过开发基于菲涅耳-基尔霍夫衍射理论的分析公式,讨论相移数字全息术中的图像形成。对于采用球形参考的一般配置,可以得出像面位置和成像倍率。研究了通过CCD离散采样所得干涉图样和数值重建对点图像质量的影响。通过数值分析点图像对最小条纹间隔与CCD像素间距之比的依赖关系。通过使用一维模型进行仿真,还研究了两点分辨率和放大率与像素数的关系。在实验中,生物物体和分辨率目标的放大图像以与传统显微镜相同的质量重建。

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