Livestock variation of linked microsatellite markers in diverse swine breeds.

摘要

A panel of 9 framework microsatellites linked to the calcium release channel (CRC) locus on pig chromosome 6 was developed from the consensus genetic map. The microsatellites were screened across groups of unrelated animals of the Yorkshire, Hampshire, Duroc, Landrace and Meishan breeds. Unique microsatellite alleles for Yorkshire, Duroc, Landrace and Meishan pigs, and statistically significant associations between breeds and allele frequencies, were found for each microsatellite. Although breed marker heterozygosities ranged from 0.0 (microsatellite S0035 in Duroc) to 0.92 (S0087 in Meishan), correspondence analysis identified microsatellite alleles uniquely associated with the Meishan or western breeds or alleles common to all breeds. An overallmarker heterozygosity of <0.70 demonstrated the need for multiple microsatellite panels to accommodate reduced within-breed differences for identification of quantitative trait loci in marker-assisted selection programmes, or parental identification incommercial breeds.