Isolation and sequencing of porcine fast skeletal muscle alkali myosin light chain 3cDNA.

摘要

A cDNA coding for alkali myosin light chain 3 (MLC3F) was isolated from a porcine skeletal muscle library. This clone has an insert of 859 bp encompassing the complete coding sequence plus the 5' and 3' untranslated regions. Computer analysis showedthat porcine MLC3F cDNA is highly homologous to the corresponding cDNAs of human, rabbit and rat. Moreover, northern analysis showed the presence of 2 bands that represent the mature mRNAs of the MLC1F and MLC3F)> isoforms, according to data observed inother species.