QTL mapping in chicken using a three generation full sib family structure of an extreme broiler X broiler cross.

摘要

A 3-generation population was created for mapping production and health traits in fowls. The F1 and F2 populations were genotyped, and phenotypes were recorded on the F3 birds. The population consisted of 10 full-sib families with a total of 476 individuals (F1 and F2), and an F3 generation consisting of over 18 000 fowls. 264 microsatellites were analysed on all F1 and F2 birds, and an additional 120 microsatellites were analysed on only 4 of the 10 families (196 birds). A linkage map of the fowlgenome containing 384 microsatellite markers was constructed by analysing the segregation of these markers. Preliminary analysis indicate a quantitative trait locus for 48-day body weight on chromosome 1. Body weight was measured on 2100 F3 fowls housedin cages, and the data were analysed by a regression interval mapping approach. Higher F-values were obtained by using a bivariate approach, indicating that differences in mean and variance of a trait measured on both sexes should be taken into account in the analysis.