Covalent Hemin-DNA Adducts for Generating a Novel Class of Artificial Heme Enzymes

摘要

An important goal of nanobiotechnology is the generation of novel biomaterials with precise control at the nanometer scale.[1] DNA oligomers play an important role as structure-directing agents in the bottom-up fabrication of nanostructured functional devices, owing to their tremendous molecular recognition capabilities. The generation of semisynthetic DNA-protein conjugates makes it possible to combine the unique properties of DNA with the almost unlimited variety of functional components of proteins, which have evolved naturally to perform highly specific catalytic turnovers, energy conversions, or translocations.[2] However, to take advantage of the distinct functionalities of DNA-protein conjugates, one must face the challenge of developing synthetic strategies that permit control over both the stoichiometry and regioselectivity of DNA-protein coupling reactions.