NADH-Induced Changes of the Nickel Coordination within the Active Site of the Soluble Hydrogenase from Alcaligenes eutrophus: XAFS Investigations on Three States Distinguishable by EPR Spectroscopy

摘要

Hydrogenases are enzymes that catalyse the reversible activation of molecular hydrogen in numerous aerobic and anaerobic microorganisms. This capability has attracted increasing interest especially in view of possible applications of the catalytic principle in industrial processes or as source for "biological hydrogen" (hydrogen technology).