The Architecture of Protein-Ligand Binding Sites Revealed through Template-Assisted Intramolecular Peptide-Peptide Interactions

摘要

Many fundamental processes of life are based on protein-peptide or protein-protein interactions.An array of discontinuous polypeptide segments within a given protein may account for the specificity and free energy change of complex formation.Characterization of the polypeptide segments that compose the ligand-binding surface requires expensive structural investigations such as multidimensional NMR spectroscopy,X-ray crystallography,and phage-displayed shotgun scanning.