High-Content Peptide Microarrays for Deciphering Kinase Specificity and Biology

摘要

Enzymatic protein phosphorylation is one of the key regulatory events in biologically important processes such as cell-cycle regulation,metabolism,and signal transduction.Protein kinases are thought to comprise the largest enzyme group in the human genome and are important targets in drug discovery.Recent estimates suggest that there are approximately 500 genes encoding protein kinases.However,only a small fraction of these enzymes have thus far been characterized and little is understood concerning the physiological functions,substrate specificity,and downstream targets of the vast majority of these enzymes.A key step in the biological characterization of kinases and in their use as drug targets is the identification of their cellular interaction partners and the identification of suitable substrates for kinase-inhibitor development.