Directed Evolution of Cyclohexanone Monooxygenases:Enantioselective Biocatalysts for the Oxidation of Prochiral Thioethers

摘要

In the preceding communication in this issue we reported that the methods of directed evolution can be used to evolve enantioselective mutants of the cyclohexanone monooxyge-nase (CHMO) from Acinetobacter sp.NCIMB 9871 (EC 1.14.13.22) as catalysts in the Baeyer-Villiger (BV) reaction of 4-hydroxycyclohexanone and other 4-substituted cyclohexanone derivatives.