Tip-Enhanced Raman Spectroscopy of Single RNA Strands:Towards a Novel Direct-Sequencing Method

摘要

The sequencing of DNA or proteins is procedurally complex and requires sophisticated analytical techniques.DNA sequencing while a very powerful method requires separation and visualization methods to recognize specific DNA fragments.'31 Furthermore,all the established methods require substantial amounts of DNA and fail to directly read the base composition of the strand.A method that utilizes the inherent information of the distinct bases present in DNA or RNA without the need of further labeling is therefore desirable.