Microfluidic Control of Fluorescence Resonance Energy Transfer: Breaking the FRET Limit

摘要

Fluorescence resonance energy transfer (FRET) is unique in providing fluorescence signals sensitive to molecular conformation, association, and separation in the range of 1-10 nm. FRET-based methods such as TaqMan and the molecular beacon assay have been widely used for the rapid detection of nucleic acids as they do not require separation of the unhybridized probes from target-probe hybrids; however, both assays require two doubly labeled probes in close proximity which are hard to optimize and synthesize.