From Native to Non-Native Two-Dimensional Protein Lattices through Underlying Hydrophilic/Hydrophobic Nanoprotrusions

摘要

Protein crystallization is a crucial step in the study of protein structure and function,as well as in biosensing.In many cases,it proceeds under ill-controlled conditions,which make it difficult to predict the outcome or feasibility.On the other hand,controlled two-dimensional(2D)protein recrystalliza-tion has been mainly accomplished through metal-ion coordination to accessible histidine residues or specific interactions with high-affinity ligands.