Photochromic Rhodamines Provide Nanoscopy with Optical Sectioning

摘要

Since the seminal work of Abbe in 1873,it has been commonly assumed that the resolution of a lens-based(far-field)light microscope is limited to about half the wavelength of the light used(≈lambda/2).However,in the mid-1990s,fluorescence microscopy concepts emerged that demonstrated that the limiting role of diffraction could be fundamentally overcome.The main hallmark of these concepts was to use the states of the fluorescent marker not just for generating the signal,but also for breaking the diffraction barrier.