Structural Basis for the Recognition of para-Benzoyl-L-phenylalanine by Evolved Aminoacyl-tRNA Synthetases

摘要

A wide array of amino acids with novel chemical and biological properties have been genetically encoded in both prokaryotic and eukaryotic organisms,which include the efficient photo-cross-linker para-benzoyl-L-phenylalanine(pBpa,Figure 1).Orthogonal tRNA/aminoacyl-tRNA syn-thetase(aaRS)pairs that selectively recognize pBpa have been evolved from both Methanococcus jannaschii(Mj)and Escherichia coli(Ec)tyrosyl-tRNA synthetases(TyrRS)in bacteria and yeast,respectively.