An Engineered Protease that Cleaves Specifically after Sulfated Tyrosine

摘要

Sulfated tyrosines are present in a wide array of proteins,such as G-protein-coupled receptors(GPCRs),anticoagulation/coagulation factors,antibodies,and bioactive peptides,such as phyllokinin,phytosulfokine,and cholecystokinin.The post-translational addition of a sulfate group to tyrosine residues on peptides and proteins is catalyzed by membrane-bound tyrosylprotein sulfotransferases(TPSTs)in the trans-Golgi network.Sulfation occurs following protein translocation to the endoplasmic reticulum,and thus,there is spatial separation between the two common forms of post-transla-tionally modified tyrosine residues,namely,phosphorylation in the cytosol,and sulfation in the extracellular space.