An Electroactive Catalytic Dynamic Substrate that Immobilizes and Releases Patterned Ligands, Proteins, and Cells

摘要

The ability to spatially and temporally control the attachment and detachment of molecules and cells on solid supports is 1 important to research areas ranging from fundamental cell biology to biomaterial development and heterogeneous catalysis. Various approaches have been developed to promote or inhibit cell attachment by altering the macroscopic properties of the materials in situ, including mechanical stretching, photochemical illumination, electrochemical modulation, and thermal activation. Alternatively, other strategies have focused on manipulating the cell-surface interactions at the molecular level by incorporating specific chemistries that can alter ligand presentation for attached cell culture through a noninvasive external switch. To access more sophisticated and complex cell behavior studies, a surface strategy that can dynamically modulate attached cell culture at the molecular level catalyti-cally, with spatial and temporal control in patterns and gradients, would greatly extend the utility of these model surfaces for a variety of cell motility, cell signaling, and cell-cell communication studies. These surfaces may also lead to the development of renewable surfaces for synthetic organic chemistry applications ranging from new solid-phase peptide synthesis resins to heterogeneous catalysis.