Solid-Phase Synthesis of Peptide Thioesters with Self-Purification

摘要

Chemical protein synthesis has facilitated studies of functional proteins by allowing the site-selective incorporation of labels,post-translational modifications,and non-proteino-genic amino acids.The most reliable access to proteins and protein domains is provided by fragment ligation techniques with native chemical ligation probably being the most frequently used method.A key requirement of the powerful native chemical ligation chemistry is the accessibility of unprotected peptide thioesters.