Ultrasensitive Label-Free Aptamer-Based Electronic Detection

摘要

Aptamers are nucleic acid ligands that bind with high affinity to their target molecules and have been shown to be particularly useful for the detection of protein analytes. As a result of their high selectivity, stability, target versatility, and convenient regeneration and modification, aptamers hold great promise for various diagnostic applications. Coupling the attractive properties of aptamers with the distinct advantages of bioelectronic transducers has received considerable recent attention. Electrochemistry offers innovative routes for interfacing aptamer interactions with the signal-generating element and for obtaining protein diagnostics in a simple, fast, and inexpensive manner. Modern electronic aptamer biosensors commonly rely on enzyme tracers, redox tags with conformational changes, or impedance detection to offer convenient measurements of nanomolar concentrations of target proteins.