A New Application of Capillary Zone Electro-phoresis: Determination of Energy Barriers of Configurationaliy Labile Chiral Compounds

摘要

Various techniques may be applied in the investigation of configurationally labile compounds. The racemates may be re-sohed into their enantiomers. and the energy barrier of enan-tiomerization investigated by chiropacal methods. Alternatives avoidingenantiornerie enrichment or separation are dynamic N MR spectroseopy. HPLC. or GC. Kinetic parameters must. however. be determined by computer simulation ot the experimental data. We describe a new procedure lor the determination of energy barriers of enanliomenzation reactions based on capillan zone electrophoresis (CZE). With this method enantiomenza.tion reactions may be investigated in the temperature range 25 deg C -45 deg C by using small amounts of racemic samples, which are typical for CZE. Subsequent computer simulation is unnecessary.