Immobilizing Enzymes: How to Create More Suitable Biocatalysts

摘要

Enzymes are versatile biocatalysts and find increasing application in many areas, including organic syntheses. The major advantages of using enymes in biocatalytic transformations are their chemo-, regio-, and stereospecificity as well as the mild reaction conditions that can be used. However, even when an enzyme is identified as being useful for a given reaction, its application is often hampered by its lack of long-term stability under process conditions, and also by difficulties in recovery and recycling. These problems can be overcome by immobilization, which provides the following advantages: ·enhanced stability, ·repeated or continuous use·easy separation from the reaction mixture·possible modulation of the catalytic properties·prevention of protein contamination in the product ·easier prevention of microbial contaminations.